Practical Considerations for the Measurement of Free Light Chains in Serum

Practical considerations for the measurement of free light chains in serum.

BACKGROUND A new immunoassay for free light chain measurements has been reported to be useful for the diagnosis and monitoring of monoclonal light chain diseases and nonsecretory myeloma. We describe experience with and some potential pitfalls of the assay. METHODS The assay was assessed for precision, sample type and stability, recovery, and harmonization of results between two analyzers on ...

Measurement of immunoglobulin free light chains in serum.

but the active/latent isoform proportions were similar (Fig. 1A). Platelet aggregation during clotting (6) may have caused these differences. MMP expression was lower in K 2 E plasma than in LH plasma [14 (3) vs 25 (4) ␮g/L; P Ͻ0.05; Fig. 1A, lane 4 vs lane 5]. The concentrations of MMP-9 forms decreased significantly with increasing amounts of K 2 E during PB collection, whereas MMP-2 was incr...

Measurement of free light chains in urine.

romantic education:reading william wordsworths the prelude in the light of the history of ideas

عصر روشنگری زمان شکل گیری ایده های مدرن تربیتی- آموزشی بود اما تاکید بیش از اندازه ی دوشاخه مهم فلسفی زمان یعنی عقل گرایی و حس گرایی بر دقت و وضوح، انسان عصر روشنگری را نسبت به دیگر تواناییهایش نابینا کرده و موجب به وجود آمدن افرادی تک بعدی شد که افتخارعقلانیتشان، تاکید شان بر تجربه فردی، به مبارزه طلبیدن منطق نیاکانشان وافسون زدایی شان از دنیا وتمام آنچه با حواس پنجگانه قابل درک نبوده و یا در ...

Quantitation of serum free light chains.

under usual artificial light conditions, and storage of unproc-essed whole blood without antico-agulants at room temperature on the bench. Additionally, we tested the effect of multiple freeze-thaw cycles. 25(OH)–vitamin D 3 measurements were performed on a Cobas E601 an-alyzer (Roche), using the vitamin D 3 (25-OH) assay as recommended by the manufacturer. Samples from each storage experiment ...